Abstract
This paper describes a specific, sensitive, semiautomated, and quantitative Hep-2 cell culture-based 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay for Bacillus cereus emetic toxin. Of nine Bacillus, Brevibacillus, and Paenibacillus species assessed for emetic toxin production, only B. cereus was cytotoxic.
MeSH terms
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Bacillus cereus / isolation & purification
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Bacillus cereus / metabolism*
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Bacterial Toxins / biosynthesis*
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Bacterial Toxins / toxicity
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Bacteriological Techniques
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Coloring Agents / metabolism
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Emetics*
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Foodborne Diseases / microbiology*
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Humans
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Tetrazolium Salts / metabolism
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Thiazoles / metabolism
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Tumor Cells, Cultured
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Vacuoles
Substances
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Bacterial Toxins
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Coloring Agents
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Emetics
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Tetrazolium Salts
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Thiazoles
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thiazolyl blue