Abstract
We localized five rpoC (beta') mutations affecting Escherichia coli RNA polymerase assembly. The Ts4, XH56, and R120 mutations changed beta' residues conserved throughout eubacteria; the JE10092 mutation occurred in the hypervariable region; rpoC1 (TsX) changed a universally conserved residue and corresponds to yeast rpb1-1. Thus, distinct, predominantly conserved beta' residues participate in interactions holding RNA polymerase together.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Conserved Sequence
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DNA-Directed RNA Polymerases / biosynthesis*
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DNA-Directed RNA Polymerases / genetics*
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DNA-Directed RNA Polymerases / metabolism
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Escherichia coli / enzymology
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Escherichia coli / genetics*
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Molecular Sequence Data
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Point Mutation*
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Protein Binding
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Sequence Homology, Amino Acid
Substances
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beta' subunit of RNA polymerase
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DNA-Directed RNA Polymerases