Tolerization of mice to Schistosoma mansoni egg antigens causes elevated type 1 and diminished type 2 cytokine responses and increased mortality in acute infection

J Immunol. 1999 Apr 1;162(7):4122-32.

Abstract

The granuloma that surrounds the Schistosoma mansoni egg is the cause of pathology in murine schistosomiasis, and its formation is driven by egg Ag-stimulated type 1 and type 2 cytokines. To determine the role of egg-driven immune responses during schistosome infection we rendered CBA/Ca mice unresponsive to schistosome eggs by combined cyclophosphamide treatment and thymectomy. In the early acute stages of schistosome infection, egg-tolerized mice suffered high mortalities. Granuloma size and deposition of collagen in the liver were significantly reduced in egg-tolerized mice. Similarly, limited granuloma responses were detected in the intestines of these mice, and this was associated with a >90% reduction in egg excretion. Histologically, egg-tolerized mice had exacerbated hepatocyte damage, with extensive microvesicular steatosis. Elevated plasma transaminase levels confirmed the damage to hepatocytes. Infected egg-tolerized mice had impaired proliferation responses to egg Ag but intact responses to worm Ag. Tolerized mice had diminished Ab responses to egg Ag and had a type 1 cytokine isotype pattern to worm Ag, with elevated IgG2a and diminished IgG1 and IgE. Egg-tolerized mice failed to down-regulate type 1 cytokines that are normally elicited during early schistosome infection. Hepatic granuloma cells from egg-tolerized mice were also type 1 cytokine dominated, with elevated frequencies of Tc1/Th1 and reduced Tc2/Th2 cells. This study demonstrates that mice tolerized to schistosome eggs have elevated type 1 cytokine responses with diminished type 2 responses and reduced anti-egg Ab during schistosome infection, and these effects are detrimental to the host.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Helminth / immunology*
  • Cytokines / biosynthesis*
  • Down-Regulation / immunology
  • Female
  • Granuloma / immunology
  • Immune Tolerance / immunology*
  • Immunity, Cellular
  • Immunoglobulins / biosynthesis
  • Liver Diseases, Parasitic / immunology
  • Mice
  • Mice, Inbred CBA
  • Ovum / immunology*
  • Schistosoma mansoni / immunology*
  • Schistosomiasis mansoni / immunology*
  • Schistosomiasis mansoni / mortality
  • Schistosomiasis mansoni / parasitology
  • Th1 Cells / immunology
  • Th1 Cells / metabolism*
  • Th2 Cells / immunology
  • Th2 Cells / metabolism*

Substances

  • Antigens, Helminth
  • Cytokines
  • Immunoglobulins