Design and syntheses of three haptens to generate catalytic antibodies that cleave amide bonds with nucleophilic catalysis

Bioorg Med Chem. 1999 Feb;7(2):279-86. doi: 10.1016/s0968-0896(98)00203-x.

Abstract

Design principles and syntheses of three haptens that were recently reported to generate amide bond cleaving catalytic antibodies are described. The hapten designs sought to induce acidic and/or basic residues in antibody binding sites via charge complementarity, and also to generate a hydrophobic binding pocket for an external phenol nucleophile. The charged yet aromatic nature of these haptens presented some unique synthetic challenges and solutions to which are described below.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amides / chemistry
  • Antibodies, Catalytic / chemistry*
  • Catalysis*
  • Haptens / chemistry*
  • Magnetic Resonance Spectroscopy
  • Models, Chemical
  • Spectrophotometry

Substances

  • Amides
  • Antibodies, Catalytic
  • Haptens