Family linkage study of the T-cell receptor genes in coeliac disease

Ital J Gastroenterol Hepatol. 1999 Apr;31(3):198-201.

Abstract

Background: The susceptibility to coeliac disease is genetically determined by possession of certain HLA DQ alleles, together with a one or more non-HLA genes. The central role of the T-cell receptor in disease pathogenesis makes the T-cell receptor genes strong candidates as disease susceptibility genes, and previous studies had provided equivocal ambiguous results.

Methods: A pedigree based genetic linkage study was used to determine if any of the T-cell receptor genes have a role in the genetic aetiology of coeliac disease. Intragenic microsatellite markers were used to study T-cell receptor alpha, beta, and delta, while gamma was studied using two flanking microsatellites D7S484 and D7S629.

Results: Conventional linkage analysis was performed using the MLINK computer package. Model-free linkage analysis was performed using MFLINK. No evidence of linkage between coeliac disease and the T-cell receptor genes was found in these pedigrees.

Conclusions: Mutations in the T-cell receptor genes are not implicated in the genetic aetiology of coeliac disease.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Case-Control Studies
  • Celiac Disease / genetics*
  • Cohort Studies
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Genes, T-Cell Receptor*
  • Genetic Linkage*
  • Genetic Markers
  • Histocompatibility Testing
  • Humans
  • Male
  • Molecular Sequence Data
  • Pedigree
  • Polymerase Chain Reaction
  • Population Surveillance
  • Sensitivity and Specificity

Substances

  • Genetic Markers