RNA-protein complexes

Curr Opin Struct Biol. 1999 Feb;9(1):66-73. doi: 10.1016/s0959-440x(99)80009-8.

Abstract

RNA-binding proteins are an extremely diverse group of proteins, reflecting the diverse functional requirements of cellular RNAs. Whereas the number of structures of RNA-binding proteins or modules is increasing at a reasonable rate, that of protein-RNA complexes increments by only a few each year. The recently determined structure of a complex from the U2 small nuclear ribonucleoprotein particle shows the subtleties of RNA stem-loop recognition by ribonucleoprotein modules. A second structure provides the first direct information on double-stranded RNA recognition by the double-stranded RNA-binding module that occurs in a variety of functionally distinct proteins. Another two new complexes concern proteins interacting with tRNA. The first is methionyl-tRNAf(Met) transformylase, which has to compete with elongation factor Tu for charged initiator tRNAMet and does so by recognising specific features of the acceptor stem of tRNAf(Met). The second is prolyl-tRNA synthetase, complexed with its cognate tRNA, that has to specifically recognise the two guanines common to all tRNA anticodons specific for proline.

Publication types

  • Review

MeSH terms

  • Amino Acid Sequence
  • Amino Acyl-tRNA Synthetases / chemistry
  • Amino Acyl-tRNA Synthetases / metabolism
  • Animals
  • Base Sequence
  • Binding Sites
  • Hydroxymethyl and Formyl Transferases / chemistry
  • Hydroxymethyl and Formyl Transferases / metabolism
  • Macromolecular Substances
  • Models, Molecular
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Protein Conformation
  • RNA / chemistry*
  • RNA / genetics
  • RNA / metabolism*
  • RNA, Double-Stranded / chemistry
  • RNA, Double-Stranded / metabolism
  • RNA, Small Nuclear / chemistry
  • RNA, Small Nuclear / metabolism
  • RNA, Transfer, Pro / chemistry
  • RNA, Transfer, Pro / metabolism
  • RNA-Binding Proteins / chemistry*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • Spliceosomes / chemistry
  • Spliceosomes / metabolism

Substances

  • Macromolecular Substances
  • RNA, Double-Stranded
  • RNA, Small Nuclear
  • RNA, Transfer, Pro
  • RNA-Binding Proteins
  • RNA
  • Hydroxymethyl and Formyl Transferases
  • methionyl-tRNA formyltransferase
  • Amino Acyl-tRNA Synthetases
  • prolyl T RNA synthetase