The enantioselectivity of the cellular deoxynucleoside kinases

J Wang; J Chattopadhyaya; S Eriksson

doi:10.1080/15257779908041567

The enantioselectivity of the cellular deoxynucleoside kinases

Nucleosides Nucleotides. 1999 Apr-May;18(4-5):807-10. doi: 10.1080/15257779908041567.

Authors

J Wang¹, J Chattopadhyaya, S Eriksson

Affiliation

¹ Department of Veterinary Medical Chemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden.

PMID: 10432682
DOI: 10.1080/15257779908041567

Abstract

Cytosolic thymidine kinase (TK1) and deoxycytidine kinase (dCK) and the mitochondrial thymidine kinase (TK2) and deoxyguanosine kinase (dGK) phosphorylate deoxynucleosides and their analogs. Recombinant human TK1 only phosphorylated beta-D Thd, but recombinant TK2, dCK and dGK all phosphorylated equally well beta-D and beta-L as well as to some extent alpha-D and alpha-L deoxynucleosides.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Deoxycytidine Kinase / metabolism*
Humans
Phosphorylation
Phosphotransferases (Alcohol Group Acceptor) / metabolism*
Recombinant Proteins / metabolism
Stereoisomerism
Thymidine Kinase / metabolism*

Substances

Recombinant Proteins
Phosphotransferases (Alcohol Group Acceptor)
deoxyguanosine kinase
Thymidine Kinase
Deoxycytidine Kinase