Binding of soluble immune complexes to fractionated Atlantic salmon (Salmo salar L.) leucocytes

Vet Immunol Immunopathol. 1999 May;68(2-4):149-57. doi: 10.1016/s0165-2427(99)00018-5.

Abstract

Binding of a fluorescent-labelled soluble immune complex to different types of Atlantic salmon leucocytes was investigated using flow cytometry. Peripheral blood leucocytes (PBL) were separated into sIg+ and sIg enriched populations by magnetic activated cell sorting, blood neutrophils were identified by electronic gating, and kidney macrophages were selected by plastic-adherence. About 60% of both sIg+ and sIg- enriched PBL, 44% of neutrophils and 34% of macrophages bound the soluble immune complexes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigen-Antibody Complex / immunology*
  • B-Lymphocytes / immunology
  • Cell Separation
  • Flow Cytometry
  • Immunoglobulins / immunology
  • Kidney / cytology
  • Leukocytes / immunology*
  • Macrophages / immunology*
  • Neutrophils / immunology*
  • Salmo salar / immunology*
  • Solubility

Substances

  • Antigen-Antibody Complex
  • Immunoglobulins