Abstract
Recent DNA sequence analysis indicates that rhesus rhadinovirus (RRV) is a member of the lymphotropic gamma-2 herpesvirus family. To determine if RRV is lymphotropic, peripheral blood mononuclear cells from naturally infected monkeys were separated by immunomagnetic bead depletion and analyzed for the presence of RRV by virus isolation and nested PCR. The recovery and consistent detection of RRV in the CD20(+)-enriched fraction clearly demonstrates that B lymphocytes are a major site of virus persistence.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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B-Lymphocytes / virology*
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DNA-Directed DNA Polymerase / genetics
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Herpesviridae Infections / blood
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Herpesviridae Infections / veterinary*
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Herpesviridae Infections / virology
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Macaca mulatta
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Monkey Diseases / blood
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Monkey Diseases / virology*
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Rhadinovirus / enzymology
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Rhadinovirus / genetics
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Rhadinovirus / isolation & purification
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Rhadinovirus / physiology*
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T-Lymphocytes / virology
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Tumor Virus Infections / blood
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Tumor Virus Infections / veterinary*
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Tumor Virus Infections / virology
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Virus Latency*
Substances
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DNA-Directed DNA Polymerase