Effect of adenosine derivatives on in vitro thrombus formation induced by shear stress

Haematologica. 1999 Aug;84(8):721-5.

Abstract

Background and objective: Shear-stress is considered to be the first event of platelet aggregation in vivo and platelet adhesion may be enhanced under pathologic conditions (e.g. arterial occlusion).

Design and methods: We wanted to test the effect of adenosine derivatives on platelet aggregation induced by shear-stress. By increasing platelet cAMP adenosine derivatives inhibit platelet activation. This in turn leads to P-selectin (CD62P) exposure, which is known to play a fundamental role in the binding of platelets to leukocytes. This gives rise to thrombus formation.

Results: The levels of cAMP (pmol/mL) prior to and after treating blood with the following compounds were respectively: PGE1 4.67+/-0.29, 9.33+/-0. 58; SP64 5.2+/-0.34, 6.83+/-0.52; 2-Cl-adenosine 5.83+/- 0.58, 7. 45+/-0.55; NECA 7.00+/-2.29, 8.00+/-1.76.

Interpretation and conclusions: High shear rate was studied using a filteraggregometer which could be a good test for analyzing what happens under physiologic conditions compared to other systems in which platelet aggregation only occurs after adding aggregating agents (Born aggregometer).

MeSH terms

  • Adenosine / analogs & derivatives
  • Adenosine / pharmacology*
  • Cyclic AMP
  • Humans
  • P-Selectin
  • Platelet Aggregation / drug effects*
  • Stress, Mechanical

Substances

  • P-Selectin
  • Cyclic AMP
  • Adenosine