Short-term effects of mineral particle sizes on cellular degradation activity after implantation of injectable calcium phosphate biomaterials and the consequences for bone substitution

Bone. 1999 Aug;25(2 Suppl):71S-74S. doi: 10.1016/s8756-3282(99)00137-4.

Abstract

This in vivo study investigated the influence of two calcium phosphate particle sizes (40-80 microm and 200-500 microm) on the cellular degradation activity associated with the bone substitution process of two injectable bone substitutes (IBS). The tested biomaterials were obtained by associating a biphasic calcium phosphate (BCP) ceramic mineral phase and a 3% aqueous solution of a cellulosic polymer (hydroxypropylmethylcellulose). Both were injected into osseous defects at the distal end of rabbit femurs for 2- and 3-week periods. Quantitative results for tartrate-resistant acid phosphatase (TRAP) cellular activity, new bone formation, and ceramic resorption were studied for statistical purposes. Positive TRAP-stained degradation cells were significantly more numerous for IBS 40-80 than IBS 200-500, regardless of implantation time. BCP degradation was quite marked during the first 2 weeks for IBS 40-80, and bone colonization occurred more extensively for IBS 40-80 than for IBS 200-500. The resorption-bone substitution process occurred earlier and faster for IBS 40-80 than IBS 200-500. Both tested IBS displayed similar biological efficiency, with conserved in vivo bioactivity and bone-filling ability. Differences in calcium phosphate particle sizes influenced cellular degradation activity and ceramic resorption but were compatible with efficient bone substitution.

MeSH terms

  • Acid Phosphatase / metabolism
  • Animals
  • Biocompatible Materials
  • Biodegradation, Environmental
  • Bone Substitutes / chemistry
  • Bone Substitutes / metabolism
  • Bone Substitutes / pharmacology*
  • Calcium Phosphates / chemistry
  • Calcium Phosphates / metabolism
  • Calcium Phosphates / pharmacology*
  • Electron Probe Microanalysis
  • Female
  • Femur / drug effects*
  • Femur / enzymology
  • Femur / pathology
  • Implants, Experimental*
  • Injections
  • Isoenzymes / metabolism
  • Lactose / analogs & derivatives*
  • Lactose / chemistry
  • Lactose / metabolism
  • Lactose / pharmacology
  • Materials Testing
  • Methylcellulose / analogs & derivatives*
  • Methylcellulose / chemistry
  • Methylcellulose / metabolism
  • Methylcellulose / pharmacology
  • Microscopy, Electron, Scanning
  • Minerals / metabolism*
  • Osseointegration* / drug effects
  • Osseointegration* / physiology
  • Oxazines
  • Particle Size
  • Rabbits
  • Tartrate-Resistant Acid Phosphatase

Substances

  • Biocompatible Materials
  • Bone Substitutes
  • Calcium Phosphates
  • Isoenzymes
  • Minerals
  • Oxazines
  • beta-tricalcium phosphate-monocalcium phosphate monohydrate-calcium sulfate hemihydrate mixture
  • Methylcellulose
  • MK 458
  • Acid Phosphatase
  • Tartrate-Resistant Acid Phosphatase
  • Lactose