A simple assay for formate dehydrogenase activity by gas chromatography-mass spectrometry

T Shiraishi; E Fukusaki; S Kajiyama; A Kobayashi

doi:10.1016/s0021-9673(99)00653-6

A simple assay for formate dehydrogenase activity by gas chromatography-mass spectrometry

J Chromatogr A. 1999 Sep 3;855(1):337-40. doi: 10.1016/s0021-9673(99)00653-6.

Authors

T Shiraishi¹, E Fukusaki, S Kajiyama, A Kobayashi

Affiliation

¹ Department of Biotechnology, Graduate School of Engineering, Osaka University, Japan.

PMID: 10514998
DOI: 10.1016/s0021-9673(99)00653-6

Abstract

A new method using GC-MS was devised for the convenient measurement of formate dehydrogenase (FDH) activity in crude tissue samples. FDH activity was detected by measuring headspace 13CO2, which was enzymically converted from [13C]formic acid. This method proved to be sensitive and simple for the estimation of FDH activity without complicated pretreatment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Formate Dehydrogenases / metabolism*
Gas Chromatography-Mass Spectrometry / methods*
Oryza / enzymology
Sensitivity and Specificity

Substances

Formate Dehydrogenases