Monocyte-derived dendritic cells as a model for the study of HIV-1 infection: productive infection and phenotypic changes during culture in human serum

Immunol Cell Biol. 1999 Oct;77(5):442-50. doi: 10.1046/j.1440-1711.1999.00853.x.

Abstract

Dendritic cells (DC) have been implicated in the initial selection for macrophage-tropic HIV-1 during transmission and in the generation of high-level virus replication during interactions with CD4 T cells. The role of DC as viral reservoirs and the extent of productive infection is unclear, but the ability to generate large numbers of DC from blood monocytes has produced a tractable model for study of DC-HIV-1 interactions. When cultured in granulocyte-macrophage colony stimulating factor and IL-4, sorted CD14+ monocytes rapidly lost phagocytic function for both 93 nm and 977 nm latex particles and developed the surface markers and function of DC. After 7 days, when returned to medium containing human serum without cytokines, some monocyte-derived dendritic cells (MDDC) became adherent, but retained the costimulatory markers CD80 and CD86 and continued to express CD83 and CD40. The MDDC stimulated allogeneic CD4 T cells, did not express new macrophage markers and remained non-phagocytic. With or without TNF-alpha, MDDC generated in cytokines were infected by macrophage and T cell-tropic virus and produced higher reverse transcriptase levels than did the autologous monocyte-derived macrophages (MDM). When added to T cells, the infected MDDC were able to infect T cells with a wider range of viral isolates than were MDM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens, CD / metabolism
  • Biomarkers
  • Blood
  • Cell Culture Techniques / methods
  • Cell Differentiation
  • Culture Media / chemistry*
  • Dendritic Cells / cytology*
  • Dendritic Cells / immunology
  • Dendritic Cells / virology*
  • Flow Cytometry
  • Granulocyte-Macrophage Colony-Stimulating Factor
  • HIV-1 / growth & development*
  • Humans
  • Immunophenotyping
  • Interleukin-4
  • Macrophages / cytology
  • Monocytes / cytology*
  • Time Factors

Substances

  • Antigens, CD
  • Biomarkers
  • Culture Media
  • Interleukin-4
  • Granulocyte-Macrophage Colony-Stimulating Factor