Training ribozymes to switch

Nat Struct Biol. 1999 Nov;6(11):992-4. doi: 10.1038/14872.

Abstract

Ribozymes that are sensitive to cyclic nucleotides have been selected in vitro. Remarkably, the cGMP-dependent ribozymes are specifically activated by a factor of 5,000 - the largest allosteric ribozyme activation by a small molecule seen to date.

Publication types

  • Comment
  • News

MeSH terms

  • Allosteric Regulation / drug effects
  • Catalysis / drug effects
  • Cyclic GMP / pharmacology*
  • Enzyme Activation / drug effects
  • Genetic Engineering / methods*
  • Nucleic Acid Conformation / drug effects*
  • Oligonucleotide Array Sequence Analysis / methods*
  • RNA, Catalytic / chemistry*
  • RNA, Catalytic / genetics
  • RNA, Catalytic / metabolism*

Substances

  • RNA, Catalytic
  • Cyclic GMP