Increased levels of epsilon and gamma isoforms of 14-3-3 proteins in cerebrospinal fluid in patients with Creutzfeldt-Jakob disease

Clin Diagn Lab Immunol. 1999 Nov;6(6):983-5. doi: 10.1128/CDLI.6.6.983-985.1999.

Abstract

We established four hybridoma cell lines producing monoclonal antibodies (MAbs) against 14-3-3 proteins. Immunoblot analysis revealed that epsilon and gamma isoforms were specifically increased in premortem cerebrospinal fluid samples from patients with sporadic Creutzfeldt-Jakob disease. Furthermore, dot immunoblot analysis showed that MAbs were more specific for native antigen than polyclonal antibodies were.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 14-3-3 Proteins
  • Antibodies, Monoclonal
  • Antibody Specificity
  • Creutzfeldt-Jakob Syndrome / cerebrospinal fluid*
  • Creutzfeldt-Jakob Syndrome / diagnosis*
  • Humans
  • Hybridomas
  • Immunoblotting
  • Isomerism
  • Proteins* / chemistry
  • Proteins* / immunology
  • Proteins* / metabolism
  • Tyrosine 3-Monooxygenase*

Substances

  • 14-3-3 Proteins
  • Antibodies, Monoclonal
  • Proteins
  • Tyrosine 3-Monooxygenase