Regions in vertebrate photoreceptor guanylyl cyclase ROS-GC1 involved in Ca(2+)-dependent regulation by guanylyl cyclase-activating protein GCAP-1

FEBS Lett. 1999 Oct 22;460(1):27-31. doi: 10.1016/s0014-5793(99)01312-5.

Abstract

The membrane bound guanylyl cyclase (GC) photoreceptor membrane GC1 (ROS-GCI) of photoreceptor cells synthesizes cGMP, the intracellular transmitter of vertebrate phototransduction. The activity of ROS-GCI is controlled by small Ca(2+)-binding proteins, named GC-activating proteins (GCAPs). We identified and characterized two short regulatory regions (M445-L456 and L503-1522) in the juxtamembrane domain (JMD) of ROS-GC1 by peptide competition and mutagenesis studies. Both regions are critical for the activation of ROS-GCI by GCAP-1.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • COS Cells
  • Calcium / pharmacology*
  • Calcium-Binding Proteins / pharmacology*
  • Cattle
  • Enzyme Activation / drug effects
  • Guanylate Cyclase / antagonists & inhibitors
  • Guanylate Cyclase / chemistry*
  • Guanylate Cyclase / genetics
  • Guanylate Cyclase-Activating Proteins
  • Molecular Sequence Data
  • Mutation
  • Peptide Library
  • Peptides / chemistry
  • Peptides / pharmacology
  • Photoreceptor Cells, Vertebrate / metabolism*
  • Receptors, Cell Surface*
  • Recombinant Proteins

Substances

  • Calcium-Binding Proteins
  • Guanylate Cyclase-Activating Proteins
  • Peptide Library
  • Peptides
  • Receptors, Cell Surface
  • Recombinant Proteins
  • guanylate cyclase 1
  • Guanylate Cyclase
  • Calcium