RNase E enzymes from rhodobacter capsulatus and Escherichia coli differ in context- and sequence-dependent in vivo cleavage within the polycistronic puf mRNA

J Bacteriol. 1999 Dec;181(24):7621-5. doi: 10.1128/JB.181.24.7621-7625.1999.

Abstract

The 5' pufQ mRNA segment and the pufLMX mRNA segment of Rhodobacter capsulatus exhibit different stabilities. Degradation of both mRNA segments is initiated by RNase E-mediated endonucleolytic cleavage. While Rhodobacter RNase E does not discriminate between the different sequences present around the cleavage sites within pufQ and pufL, Escherichia coli RNase E shows preference for the sequence harboring more A and U residues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins*
  • Endoribonucleases / metabolism*
  • Escherichia coli / enzymology*
  • Light-Harvesting Protein Complexes*
  • Nucleic Acid Conformation
  • Photosynthetic Reaction Center Complex Proteins / genetics
  • Photosynthetic Reaction Center Complex Proteins / metabolism
  • RNA, Messenger / metabolism*
  • Rhodobacter capsulatus / enzymology*

Substances

  • Bacterial Proteins
  • Light-Harvesting Protein Complexes
  • Photosynthetic Reaction Center Complex Proteins
  • PufL protein, Bacteria
  • RNA, Messenger
  • Endoribonucleases
  • ribonuclease E