Aptamers containing thymidine 3'-O-phosphorodithioates: synthesis and binding to nuclear factor-kappaB

Bioorg Med Chem Lett. 1999 Dec 6;9(23):3357-62. doi: 10.1016/s0960-894x(99)00600-9.

Abstract

Aptamers targeting NF-kappaB containing thymidine 3'-O-phosphorodithioates in selected positions of an oligonucleotide duplex were synthesized. Binding affinities to NF-kappaB varied with the number and positions of the dithioate backbone substitutions. One of the aptamers showed specific binding to a single NF-kappaB dimer in cell culture extracts.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Magnetic Resonance Spectroscopy
  • NF-kappa B / metabolism*
  • Oligonucleotides / chemical synthesis*
  • Oligonucleotides / chemistry
  • Oligonucleotides / metabolism
  • Organothiophosphorus Compounds / analysis*

Substances

  • NF-kappa B
  • Oligonucleotides
  • Organothiophosphorus Compounds