Abstract
The ability of variants of the human respiratory syncytial virus (HRSV) phosphoprotein (P protein) to support RNA transcription and replication has been studied by using HRSV-based subgenomic replicons. The serine residues normally phosphorylated in P during HRSV infection have been replaced by other residues. The results indicate that the bulk of phosphorylation of P (98%) is not essential for viral RNA transcription or replication but that phosphorylation can modulate these processes.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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HN Protein*
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Humans
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Phosphoproteins / metabolism*
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Phosphorylation
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RNA, Messenger / genetics
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RNA, Messenger / metabolism*
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RNA, Viral / genetics
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RNA, Viral / metabolism*
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Replicon
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Respiratory Syncytial Viruses / genetics
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Respiratory Syncytial Viruses / metabolism*
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Transcription, Genetic*
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Viral Envelope Proteins
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Viral Proteins / metabolism*
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Virus Replication
Substances
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HN Protein
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Phosphoproteins
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RNA, Messenger
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RNA, Viral
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Viral Envelope Proteins
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Viral Proteins
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attachment protein G