Based on mapping observations with ASCA, an unusual hot region with a spatial extent of 1 deg2 was discovered between M87 and M49 at a center coordinate of R.A.=12h27m36s and decl.=9&j0;18' (J2000). The X-ray emission from the region has a 2-10 keV flux of 1x10-11 ergs s-1 cm-2 and a temperature of kT greater, similar4 keV, which is significantly higher than that in the surrounding medium of approximately 2 keV. The internal thermal energy in the hot region is estimated to be VnkT approximately 1060 ergs with a gas density of approximately 10-4 cm-3. A power-law spectrum with a photon index of 1.7-2.3 is also allowed by the data. The hot region suggests there is an energy input due to a shock that is probably caused by the motion of the gas associated with M49, infalling toward the M87 cluster with a velocity greater, similar1000 km s-1.