Complete sequence analysis of the A*1103 allele

Tissue Antigens. 2000 Jan;55(1):68-70. doi: 10.1034/j.1399-0039.2000.550113.x.

Abstract

Here we report the full-length sequence of a novel A*11 variant. The variant was identified by ARMS-PCR and serology, the sequence was confirmed by cloning and subsequent sequencing. This variant, A*1103, found in a family of oriental origin, resembles the A*1101 sequence in exon 2 but differs in exon 3 with regard to codons 151 and 152. The polymorphism's result in two amino acid substitutions (one conserved (His->Arg), one introducing a negative charge (Ala->Glu)) located in the alpha2 helical region. The arginine at amino acid position 151 is rare amongst Alocus alleles and is besides A*1103 only observed in A*29 variants, the glutamine at amino acid position 152 is shared with A*0301, A*25, *26, *34 variants and the A*02 subtypes A*0203, *0213 and *0226. In fact, the amino acid motif comprising codons 151 and 152 is unique to A*1103 among Alocus alleles, but is common to C-locus alleles.

MeSH terms

  • Alleles*
  • Amino Acid Substitution
  • Base Sequence
  • Codon
  • DNA / analysis
  • HLA-A Antigens / genetics*
  • HLA-A11 Antigen
  • Humans
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Polymorphism, Genetic*
  • Sequence Analysis, DNA

Substances

  • Codon
  • HLA-A Antigens
  • HLA-A11 Antigen
  • DNA

Associated data

  • GENBANK/Y17224