The reduction of the Rieske iron-sulfur cluster in naphthalene dioxygenase by X-rays

J Inorg Biochem. 2000 Jan 15;78(1):83-7. doi: 10.1016/s0162-0134(99)00213-5.

Abstract

Naphthalene 1,2 dioxygenase (NDO) displays characteristic UV-Vis spectra depending on the oxidation state of the Rieske center. Investigations on crystals of NDO grown for X-ray diffraction experiments showed spectra characteristic of the oxidized form. Crystals reduced in an anaerobic glovebox using sodium-dithionite showed a characteristic reduced spectrum. Spectra of crystals (cooled to 100 K) after being exposed to X-rays for data collection showed spectra corresponding to a reduced Rieske iron center, demonstrating the ability of X-rays to change the oxidation state of the Rieske iron-sulfur cluster in NDO.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Crystallization
  • Dioxygenases
  • Escherichia coli / enzymology
  • Iron / metabolism
  • Iron / radiation effects*
  • Iron-Sulfur Proteins / chemistry
  • Iron-Sulfur Proteins / metabolism
  • Iron-Sulfur Proteins / radiation effects*
  • Microspectrophotometry
  • Multienzyme Complexes / chemistry
  • Multienzyme Complexes / metabolism
  • Multienzyme Complexes / radiation effects*
  • Oxidation-Reduction
  • Oxygenases / chemistry
  • Oxygenases / metabolism
  • Oxygenases / radiation effects*
  • Sulfur / metabolism
  • Sulfur / radiation effects*
  • Temperature
  • Time Factors
  • X-Rays

Substances

  • Iron-Sulfur Proteins
  • Multienzyme Complexes
  • Sulfur
  • Iron
  • Oxygenases
  • Dioxygenases
  • naphthalene dioxygenase