Abstract
Pulsed-field gel electrophoresis, ribotyping, and fingerprinting analysis of 22 invasive isolates of multidrug-resistant (MDR) pneumococci from Korea showed that 59 to 82% were genetically related. DNA sequencing of the PBP 2B gene showed relatively uniform alterations in nucleotides (5.4 to 7.8%) and amino acids (3.0 to 4. 3%), while Asn-276-->Lys, Arg-285-->Cys and Ser-305-->Phe substitutions were unique to Korean MDR strains, suggesting the spread of a few epidemic clones of resistant pneumococci within Korea.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins*
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Bacterial Typing Techniques
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Carrier Proteins / genetics
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Deoxyribonucleases, Type II Site-Specific / metabolism
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Drug Resistance, Microbial
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Drug Resistance, Multiple*
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Electrophoresis, Gel, Pulsed-Field
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Hexosyltransferases*
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Humans
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Korea / epidemiology
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Muramoylpentapeptide Carboxypeptidase / genetics
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Penicillin-Binding Proteins
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Peptidyl Transferases*
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Pneumococcal Infections / epidemiology
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Pneumococcal Infections / microbiology*
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Sequence Analysis, DNA
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Streptococcus pneumoniae / classification
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Streptococcus pneumoniae / drug effects
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Streptococcus pneumoniae / genetics*
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Streptococcus pneumoniae / isolation & purification*
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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Carrier Proteins
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Penicillin-Binding Proteins
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Peptidyl Transferases
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Hexosyltransferases
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CAGCTG-specific type II deoxyribonucleases
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Deoxyribonucleases, Type II Site-Specific
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Muramoylpentapeptide Carboxypeptidase
Associated data
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GENBANK/AF180878
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GENBANK/AF180879
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GENBANK/AF180880
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GENBANK/AF180881
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GENBANK/AF180882
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GENBANK/AF180883
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GENBANK/AF180884
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GENBANK/AF180885
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GENBANK/AF180886