Structural analysis of the core region of lipopolysaccharides from Proteus mirabilis serotypes O6, O48 and O57

Eur J Biochem. 2000 Apr;267(8):2439-46. doi: 10.1046/j.1432-1327.2000.01262.x.

Abstract

The structure of lipid A core region of the lipopolysaccharides (LPS) from Proteus mirabilis serotypes O6, O57 and O48 was determined using NMR, MS and chemical analysis of the oligosaccharides, obtained by mild acid hydrolysis, alkaline deacylation, and deamination of LPS: [see text for structure]. Incomplete substitutions are indicated by bold italic type. All sugars are present in pyranose form, alpha-Hep is the residue of L-glycero-alpha-D-manno-Hep, alpha-DD-Hep is the residue of D-glycero-alpha-D-manno-Hep, L-Ara4N is 4-amino-4-deoxy-L-arabinose, Qui4NAlaAla is the residue of 4-N-(L-alanyl-L-alanyl)-4-amino-4,6-dideoxyglucose. All sugars except L-Ara4N have D-configuration. beta-GalA* is partially present in the form of amide with 1,4-diaminobutane (putrescine)-HN(CH2)4NH2 or spermidine-HN(CH2)3NH(CH2)4NH2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Lipopolysaccharides / chemistry*
  • Magnetic Resonance Spectroscopy
  • Molecular Sequence Data
  • O Antigens / chemistry*
  • O Antigens / immunology
  • Oligosaccharides / chemistry
  • Polysaccharides, Bacterial / chemistry*
  • Proteus mirabilis / chemistry*
  • Proteus mirabilis / immunology
  • Proteus mirabilis / pathogenicity
  • Serotyping

Substances

  • Lipopolysaccharides
  • O Antigens
  • Oligosaccharides
  • Polysaccharides, Bacterial