Mechanism of ribosome RNA apurinic site specific lyase

Nucleic Acids Symp Ser. 1999:(42):257-8. doi: 10.1093/nass/42.1.257.

Abstract

A new enzyme, which we named ribosome RNA apurinic site specific lyase (RALyase), has been characterized. The enzyme specifically cleaves a phosphodiester bond at the apurinic site in the sarcin/ricin domain of 28S rRNA in ribosomes. The cut ends of wheat 28S rRNA were determined as 5'---GUACG-alpha-hydroxy-alpha, beta-unsaturated aldehyde and pGAGGA---3' for the 3' fragment, demonstrating that the enzyme catalyzes the beta-elimination reaction.

MeSH terms

  • Apurinic Acid
  • Base Sequence
  • Catalysis
  • Endoribonucleases / genetics
  • Endoribonucleases / metabolism*
  • Fungal Proteins*
  • Kinetics
  • Plant Lectins
  • RNA, Ribosomal, 28S / chemistry
  • RNA, Ribosomal, 28S / metabolism*
  • Ribosomes / metabolism
  • Ricin / genetics
  • Substrate Specificity
  • Triticum

Substances

  • Fungal Proteins
  • Plant Lectins
  • RNA, Ribosomal, 28S
  • Apurinic Acid
  • alpha-sarcin
  • Ricin
  • Endoribonucleases
  • ribosomal RNA apurinic site specific lyase