On the oxygenation-dependent (129)Xe T (1) in blood

NMR Biomed. 2000 Jun;13(4):234-7. doi: 10.1002/1099-1492(200006)13:4<234::aid-nbm632>3.0.co;2-k.

Abstract

The spin-lattice relaxation time, T(1), of hyperpolarized (129)Xe in blood is sensitive to blood oxygenation. In particular, it has been shown that (129)Xe T(1) is shorter in venous blood than in arterial blood. We have studied the T(1) of hyperpolarized (129)Xe dissolved in human blood as a function of blood oxygenation level, sO(2), in the physiological oxygenation range. We show that the (129)Xe relaxation rate, T(1)(-1), varies in a nonlinear fashion as a function of sO(2). This finding suggests that direct interaction of xenon with the paramagnetic heme group of deoxyhemoglobin is not the dominant oxygenation-dependent relaxation mechanism for (129)Xe in blood. These results corroborate the idea that the oxygenation-dependence of (129)Xe T(1) is determined by conformational changes of hemoglobin induced by oxygen binding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbon Dioxide / blood
  • Humans
  • Hydrogen-Ion Concentration
  • Nuclear Magnetic Resonance, Biomolecular
  • Oxygen / blood*
  • Oxyhemoglobins / chemistry
  • Oxyhemoglobins / metabolism
  • Partial Pressure
  • Protein Conformation
  • Xenon / blood*
  • Xenon Isotopes

Substances

  • Oxyhemoglobins
  • Xenon Isotopes
  • Carbon Dioxide
  • Xenon
  • Oxygen