Abstract
Cross-linking of the B cell Ag receptor (BCR) induces the tyrosine phosphorylation of multiple cellular substrates, including phospholipase C (PLC)-gamma 2, which is involved in the activation of the phosphatidylinositol pathway. To assess the importance of PLC-gamma 2 in murine lymphopoiesis, the PLC-gamma 2 gene was inducibly ablated by using IFN-regulated Cre recombinase. Mice with a neonatally induced loss of PLC-gamma 2 function displayed reduced numbers of mature conventional B cells and peritoneal B1 cells and defective responses in vitro to BCR stimulation and in vivo to immunization with thymus-independent type II Ags. In contrast, T cell development and TCR-mediated proliferation were normal. Taken together, PLC-gamma 2 is a critical component of BCR signaling pathways and is required to promote B cell development.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Surface / analysis
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Antigens, T-Independent / immunology
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B-Lymphocytes / cytology*
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B-Lymphocytes / enzymology*
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B-Lymphocytes / immunology
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B-Lymphocytes / metabolism
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Calcium / metabolism
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Cell Differentiation / genetics
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Cell Differentiation / immunology
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Ficoll / analogs & derivatives*
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Ficoll / immunology
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Gene Deletion
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Haptens / immunology
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Hemocyanins / immunology
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Immunoglobulin M / biosynthesis
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Isoenzymes / genetics
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Isoenzymes / physiology*
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Lymphocyte Activation / genetics
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Lymphocyte Subsets / cytology
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Lymphocyte Subsets / immunology
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Mice
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Mice, Knockout
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Phospholipase C gamma
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T-Lymphocytes / cytology
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T-Lymphocytes / immunology
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Trinitrobenzenes / immunology
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Type C Phospholipases / genetics
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Type C Phospholipases / physiology*
Substances
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Antigens, Surface
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Antigens, T-Independent
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Haptens
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Immunoglobulin M
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Isoenzymes
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TNP-ficoll
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Trinitrobenzenes
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trinitrophenyl keyhole limpet hemocyanin
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Ficoll
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Hemocyanins
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Type C Phospholipases
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Phospholipase C gamma
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Calcium