Immunological characterization of 130 kDa phospholipase C-beta 4 isozyme in rat cerebellar Purkinje cells

Neurosci Lett. 2000 Sep 29;292(1):9-12. doi: 10.1016/s0304-3940(00)01419-1.

Abstract

We have developed a monoclonal antibody, beta4-22-9 that recognizes an epitope on pleckstrin homology (PH) domain (1-136 amino acids) located at the amino terminus of rat 130 kDa phospholipase C-beta4 (PLC-beta4). Tissue distribution of 130 kDa PLC-beta4 was determined with this monoclonal antibody. One hundred and thirty kiloDalton PLC-beta4 was exclusively expressed in the nervous tissues including cerebellum and cerebrum, especially at very high levels in cerebellum. The expression of 130 kDa PLC-beta4 in cerebellum was gradually increased from 5 days after birth to adulthood. In the adult cerebellum, especially high level of 130 kDa PLC-beta4 was localized on the periphery of Purkinje cells in the patch-like pattern. These data implies that 130 kDa PLC-beta4 may a role in cerebellar Purkinje cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / chemistry
  • Blood Platelets / chemistry
  • Blood Proteins / chemistry
  • Blood Proteins / immunology
  • Cerebellum / chemistry
  • Cerebellum / cytology
  • Cerebellum / enzymology*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Isoenzymes / chemistry*
  • Isoenzymes / immunology
  • Mice
  • Mice, Inbred BALB C
  • Molecular Weight
  • Phospholipase C beta
  • Phosphoproteins / chemistry
  • Phosphoproteins / immunology
  • Protein Structure, Tertiary
  • Purkinje Cells / enzymology*
  • Rats
  • Rats, Sprague-Dawley
  • Sequence Homology, Amino Acid
  • Type C Phospholipases / chemistry*
  • Type C Phospholipases / immunology

Substances

  • Antibodies, Monoclonal
  • Blood Proteins
  • Isoenzymes
  • Phosphoproteins
  • platelet protein P47
  • Type C Phospholipases
  • Phospholipase C beta
  • Plcb4 protein, mouse
  • Plcb4 protein, rat