Abstract
An in silico scan of the partially completed genome sequence of Bordetella pertussis and analyses of transcriptional fusions generated with a new integrational vector were used to identify new potential virulence genes. The genes encoding a putative siderophore receptor, adhesins, and an autotransporter protein appeared to be regulated in a manner similar to Bordetella virulence genes by the global virulence regulator BvgAS. In contrast, the gene encoding a putative intimin-like protein appeared to be repressed under conditions of virulence.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Base Sequence
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Bordetella pertussis / genetics*
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Bordetella pertussis / pathogenicity*
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Drug Resistance, Microbial / genetics
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Gene Expression Regulation, Bacterial*
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Gene Silencing
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Genes, Bacterial
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Gentamicins
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Molecular Sequence Data
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Recombinant Fusion Proteins / biosynthesis
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Recombinant Fusion Proteins / genetics
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Suppression, Genetic
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Transcription, Genetic
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Virulence / genetics*
Substances
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Bacterial Proteins
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Gentamicins
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Recombinant Fusion Proteins