Abstract
The effect of AMP-activated protein kinase (AMPK) in the regulation of the phosphoenolpyruvate carboxykinase (PEPCK) gene expression was studied in isolated rat hepatocytes. Activation of AMPK by AICAR counteracted the inhibitory effect of glucose on the PEPCK gene expression, both at the mRNA and the transcriptional levels. It is proposed that a target for AMPK is involved in the inhibitory effect of glucose on PEPCK gene transcription.
MeSH terms
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AMP-Activated Protein Kinases
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Adenosine Monophosphate / physiology*
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Aminoimidazole Carboxamide / analogs & derivatives
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Aminoimidazole Carboxamide / pharmacology
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Animals
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Enzyme Activation / drug effects
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Enzyme Activation / genetics
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Gene Expression Regulation / drug effects*
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Glucose / antagonists & inhibitors
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Glucose / pharmacology*
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Hypoglycemic Agents / pharmacology
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Liver / cytology
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Liver / drug effects
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Liver / enzymology*
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Liver / metabolism
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Male
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Multienzyme Complexes / physiology*
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Phosphoenolpyruvate Carboxykinase (GTP) / antagonists & inhibitors*
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Phosphoenolpyruvate Carboxykinase (GTP) / biosynthesis
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Phosphoenolpyruvate Carboxykinase (GTP) / genetics*
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Protein Serine-Threonine Kinases / physiology*
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RNA, Messenger / metabolism
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Rats
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Rats, Wistar
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Ribonucleotides / pharmacology
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Transcription, Genetic / drug effects
Substances
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Hypoglycemic Agents
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Multienzyme Complexes
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RNA, Messenger
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Ribonucleotides
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Aminoimidazole Carboxamide
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Adenosine Monophosphate
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Protein Serine-Threonine Kinases
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AMP-Activated Protein Kinases
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Phosphoenolpyruvate Carboxykinase (GTP)
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AICA ribonucleotide
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Glucose