Although electrophoretic-based DNA sequencing technology has been in place for more than 10 years, continued advances in the basic separation science, detection methodologies, automation and sample preparation promise to keep this technology in the forefront of genetic analysis. As the demands for sequence information moves from de novo whole genome analysis to more routine, comparative sequencing of known genes, we are confident that the technology will continue to evolve and will adapt to the demands of the scientific and commercial community.