Assessment of postprandial hepatic glycogen synthesis from uridine diphosphoglucose kinetics in obese and lean non-diabetic subjects

Int J Obes Relat Metab Disord. 2000 Oct;24(10):1297-302. doi: 10.1038/sj.ijo.0801386.

Abstract

Background: Obese patients are frequently characterized by insulin resistance and decreased insulin-mediated glycogen synthesis in skeletal muscle. Whether they also have impaired postprandial hepatic glycogen synthesis remains unknown.

Aim: To determine whether postprandial hepatic glycogen synthesis is decreased in obese patients compared to lean subjects.

Methods: Lean and obese subjects with impaired glucose tolerance were studied over 4h after ingestion of a glucose load. Hepatic uridine diphosphoglucose kinetics were assessed using 13C-galactose infusion, with monitoring of urinary acetaminophen-glucuronide isotopic enrichment to estimate hepatic glycogen kinetics.

Results: Estimated net hepatic glycogen synthesis amounted to 18.6 and 22.6% of the ingested load in lean and obese subjects, respectively.

Conclusion: Postprandial hepatic glycogen metabolism is not impaired in non-diabetic obese subjects.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Aged
  • Blood Glucose / analysis
  • Fatty Acids, Nonesterified / blood
  • Female
  • Glucose Intolerance / complications
  • Glucose Intolerance / metabolism
  • Humans
  • Insulin / blood
  • Insulin Resistance
  • Liver / metabolism*
  • Liver Glycogen / biosynthesis*
  • Male
  • Middle Aged
  • Obesity / complications
  • Obesity / metabolism*
  • Postprandial Period
  • Time Factors
  • Uridine Diphosphate Glucose / metabolism
  • Uridine Diphosphate Glucose / pharmacokinetics*

Substances

  • Blood Glucose
  • Fatty Acids, Nonesterified
  • Insulin
  • Liver Glycogen
  • Uridine Diphosphate Glucose