The SUR2 gene of Arabidopsis thaliana encodes the cytochrome P450 CYP83B1, a modulator of auxin homeostasis

Proc Natl Acad Sci U S A. 2000 Dec 19;97(26):14819-24. doi: 10.1073/pnas.260502697.

Abstract

Genetic screens have been performed to identify mutants with altered auxin homeostasis in Arabidopsis. A tagged allele of the auxin-overproducing mutant sur2 was identified within a transposon mutagenized population. The SUR2 gene was cloned and shown to encode the CYP83B1 protein, which belongs to the large family of the P450-dependent monooxygenases. SUR2 expression is up-regulated in sur1 mutants and induced by exogenous auxin in the wild type. Analysis of indole-3-acetic acid (IAA) synthesis and metabolism in sur2 plants indicates that the mutation causes a conditional increase in the pool size of IAA through up-regulation of IAA synthesis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Arabidopsis / enzymology*
  • Arabidopsis / genetics
  • Arabidopsis / metabolism
  • Arabidopsis Proteins
  • Cytochrome P-450 Enzyme System / genetics
  • Cytochrome P-450 Enzyme System / metabolism*
  • DNA, Plant / isolation & purification
  • Gene Expression Regulation
  • Genes, Plant
  • Homeostasis
  • Hydrogen-Ion Concentration
  • Indoleacetic Acids / metabolism*
  • Indoles / metabolism
  • Mixed Function Oxygenases / genetics
  • Mixed Function Oxygenases / metabolism*
  • Mutagenesis, Insertional
  • Phenotype
  • Plant Roots
  • Polymerase Chain Reaction / methods
  • Seeds / metabolism

Substances

  • Arabidopsis Proteins
  • DNA, Plant
  • Indoleacetic Acids
  • Indoles
  • indoleacetic acid
  • indole
  • Cytochrome P-450 Enzyme System
  • Mixed Function Oxygenases
  • CYP83A1 protein, Arabidopsis