Lipid oxidation in blood plasma of patients with neurological disorders

Brain Res Bull. 2000 Nov 1;53(4):437-43. doi: 10.1016/s0361-9230(00)00375-0.

Abstract

Nuclear magnetic resonance (NMR) spectra of blood plasma lipids from lyophilized plasma samples from patients with neurological disorders stored for several weeks in an evacuated exsiccator show characteristic differences compared to freshly lyophilized plasma samples. The main differences concern the unsaturated fatty acids, e.g., the extent of unsaturation and their structural composition. The total amount of double bond signals of unsaturated fatty acids are noticeably reduced in intensity and new signals arise from conjugated double bonds. These signals can be assigned to keto-octadecadienoic acid (KODE) or hydroxy-octadecadienoic acid (HODE). The proton and carbon NMR chemical shifts and their structural assignment to the main molecular components are given. Whereas the KODE and HODE signals occur only as storage artifacts in the spectra, we have found small amounts of 9,11-octadecadienoic acid also in fresh blood plasma of controls. Its concentration is about 60 microM. In two-dimensional H,H total correlation spectroscopy spectra also a very low amount (6-7 microM) of 13-HODE can be detected.

MeSH terms

  • Humans
  • Linoleic Acids / blood*
  • Linoleic Acids, Conjugated*
  • Lipid Peroxidation*
  • Lipid Peroxides / blood*
  • Magnetic Resonance Spectroscopy
  • Nervous System Diseases / blood*
  • Reference Values

Substances

  • Linoleic Acids
  • Linoleic Acids, Conjugated
  • Lipid Peroxides
  • 9,11-linoleic acid