Abstract
The requirement for processing glycolipid antigens in T cell recognition was examined with mouse CD1d-mediated responses to glycosphingolipids (GSLs). Although some disaccharide GSL antigens can be recognized without processing, the responses to three other antigens, including the disaccharide GSL Gal(alpha1-->2)GalCer (Gal, galactose; GalCer, galactosylceramide), required removal of the terminal sugars to permit interaction with the T cell receptor. A lysosomal enzyme, alpha-galactosidase A, was responsible for the processing of Gal(alpha1-->2)GalCer to generate the antigenic monosaccharide epitope. These data demonstrate a carbohydrate antigen processing system analogous to that used for peptides and an ability of T cells to recognize processed fragments of complex glycolipids.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Motifs
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Animals
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Anti-Bacterial Agents / pharmacology
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Antigen Presentation*
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Antigen-Presenting Cells / immunology
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Antigens, CD1 / chemistry
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Antigens, CD1 / immunology*
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Antigens, CD1 / metabolism
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Antigens, CD1d
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Carbohydrate Conformation
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Cytokines / biosynthesis
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Epitopes / immunology
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Galactosylceramides / chemistry
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Galactosylceramides / immunology*
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Galactosylceramides / metabolism*
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Lysosomes / enzymology
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Macrolides*
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Mice
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Mice, Inbred C57BL
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Protein Transport
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Receptors, Antigen, T-Cell / immunology
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T-Lymphocytes / immunology*
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Transfection
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Tumor Cells, Cultured
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alpha-Galactosidase / metabolism*
Substances
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Anti-Bacterial Agents
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Antigens, CD1
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Antigens, CD1d
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Cytokines
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Epitopes
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Galactosylceramides
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Macrolides
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Receptors, Antigen, T-Cell
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concanamycin A
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bafilomycin A1
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alpha-Galactosidase