Expression, extracellular secretion, and immunogenicity of the Plasmodium falciparum sporozoite surface protein 2 in Salmonella vaccine strains

Infect Immun. 2001 Feb;69(2):1192-8. doi: 10.1128/IAI.69.2.1192-1198.2001.

Abstract

Deleting transmembrane alpha-helix motifs from Plasmodium falciparum sporozoite surface protein (SSP-2) allowed its secretion from Salmonella enterica serovar Typhimurium SL3261 and S. enterica serovar Typhi CVD 908-htrA by the Hly type I secretion system. In mice immunized intranasally, serovar Typhimurium constructs secreting SSP-2 stimulated greater gamma interferon splenocyte responses than did nonsecreting constructs (P = 0.04).

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Protozoan / immunology*
  • Female
  • Immunization
  • Lymphocyte Activation
  • Malaria Vaccines / immunology*
  • Mice
  • Mice, Inbred C57BL
  • Molecular Weight
  • Plasmodium falciparum / immunology*
  • Protozoan Proteins / chemistry
  • Protozoan Proteins / genetics
  • Protozoan Proteins / immunology*
  • Salmonella / genetics*
  • T-Lymphocytes, Cytotoxic / immunology
  • Vaccines, DNA / immunology

Substances

  • Antigens, Protozoan
  • Malaria Vaccines
  • Protozoan Proteins
  • Vaccines, DNA
  • sporozoite-surface protein 2, Plasmodium yoelli