Crystallization of full-length CysB of Klebsiella aerogenes, a LysR-type transcriptional regulator

Acta Crystallogr D Biol Crystallogr. 2001 Feb;57(Pt 2):260-2. doi: 10.1107/s0907444900016851.

Abstract

CysB is a positive regulator of transcription of genes involved in cysteine biosynthesis in Gram-negative bacteria and belongs to the large family of LysR-type transcriptional regulators. The full-length protein from Klebsiella aerogenes has been crystallized from solutions containing PEG 8000 in the presence and in the absence of the inducer N-acetylserine by the method of vapour diffusion in hanging drops. For the complexed protein different crystal forms appear in the same drops.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry*
  • Bacterial Proteins / isolation & purification
  • Crystallization
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / isolation & purification
  • Dimerization
  • Enterobacter aerogenes / genetics
  • Polyethylene Glycols
  • Protein Conformation
  • Serine / analogs & derivatives*
  • Transcription Factors / chemistry
  • Transcription, Genetic

Substances

  • Bacterial Proteins
  • CysB protein, Bacteria
  • DNA-Binding Proteins
  • Transcription Factors
  • Polyethylene Glycols
  • Serine
  • LysR protein, Bacteria
  • N-acetylserine