15N NMR relaxation as a probe for helical intrinsic propensity: the case of the unfolded D2 domain of annexin I

J Biomol NMR. 2001 Jan;19(1):3-18. doi: 10.1023/a:1008390606077.

Abstract

The isolated D2 domain of annexin I is unable to adopt a tertiary fold but exhibits both native and non-native residual structures. It thus constitutes an attractive model for the investigation of dynamics of partially folded states in the context of protein folding and stability. 15N relaxation parameters of the D2 domain have been acquired at three different magnetic fields, 500, 600 and 800 MHz. This enables the estimation of the contribution of conformational exchange to the relaxation parameters on the micro- to millisecond time scale, thus providing a suitable data set for the description of motions on the pico- and nanosecond time scale. The analysis of the seven spectral densities obtained (J(0), J(50 MHz), J(60 MHz), J(80 MHz), <J(500 MHz)>, <J(600 MHz)>, <J(800 MHz)>) provides complementary and meaningful results on the conformational features of the D2 domain structure previously depicted by chemical shift and NOE data. Especially, residual helix segments exhibit distinct dynamical behaviors that are related to their intrinsic helical propensity. Beside the spectral density analysis, a series of models derived from the Lipari and Szabo model-free approach are investigated. Two models containing three parameters are able to reproduce equally well the experimental data within experimental errors but provide different values of order parameters and correlation times. The inability to find a unique model to describe the data emphasizes the difficulty to use and interpret the model-free parameters in the case of partially or fully unfolded proteins consisting of a wide range of interconverting conformers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Annexin A1 / chemistry*
  • Magnetics
  • Models, Molecular
  • Nitrogen Isotopes
  • Nonlinear Dynamics
  • Nuclear Magnetic Resonance, Biomolecular / methods*
  • Peptide Fragments / chemistry
  • Protein Folding
  • Protein Structure, Secondary
  • Protein Structure, Tertiary

Substances

  • Annexin A1
  • Nitrogen Isotopes
  • Peptide Fragments