Abstract
To detect the molecules that interact with infectious bursal disease virus (IBDV), the chicken B lymphoblastoid cell line, LSCC-BK3, which is permissive for virulent IBDV infection was investigated. The sodium dodecyl sulfate-solubilized plasma membrane fraction from the cells was subjected to a virus overlay protein binding assay. The IBDV specifically bound to proteins in LSCC-BK3 plasma membranes with molecular weights of 70, 82 and 110 kDa. This is the first report to demonstrate cellular molecules that interact with virulent IBDV.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Birnaviridae Infections / immunology
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Birnaviridae Infections / veterinary*
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Birnaviridae Infections / virology
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Cell Membrane / virology
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Cells, Cultured
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Chickens
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Infectious bursal disease virus / immunology
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Infectious bursal disease virus / metabolism
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Infectious bursal disease virus / pathogenicity*
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Membrane Proteins / immunology
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Membrane Proteins / isolation & purification*
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Membrane Proteins / metabolism
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Molecular Weight
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Poultry Diseases / immunology
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Poultry Diseases / virology*
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Protein Binding / immunology