Abstract
By combining photoconversion of FM1-43-stained vesicles and electron microscopy of hippocampal synapses, we find evidence that the population of morphologically docked synaptic vesicles corresponds to the release-ready neurotransmitter quanta. Furthermore, those synaptic vesicles that are participating in cycles of exo- and endocytosis tend to be closer to the active zone than vesicles that are being held in reserve.
MeSH terms
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Animals
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Cells, Cultured
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Fluorescent Dyes / analysis
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Hippocampus / chemistry
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Hippocampus / cytology*
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Hippocampus / ultrastructure
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Image Processing, Computer-Assisted
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Neurons / chemistry*
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Neurons / ultrastructure
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Neurotransmitter Agents / analysis*
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Pyridinium Compounds / analysis
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Quaternary Ammonium Compounds / analysis
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Rats
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Regression Analysis
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Synapses / ultrastructure
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Synaptic Vesicles / chemistry*
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Synaptic Vesicles / ultrastructure*
Substances
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FM1 43
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Fluorescent Dyes
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Neurotransmitter Agents
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Pyridinium Compounds
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Quaternary Ammonium Compounds