Reduced skeletal muscle calpain-10 transcript level is due to a cumulative decrease in major isoforms

X Yang; R E Pratley; L J Baier; Y Horikawa; G I Bell; C Bogardus; P A Permana

doi:10.1006/mgme.2001.3171

Reduced skeletal muscle calpain-10 transcript level is due to a cumulative decrease in major isoforms

Mol Genet Metab. 2001 May;73(1):111-3. doi: 10.1006/mgme.2001.3171.

Authors

X Yang¹, R E Pratley, L J Baier, Y Horikawa, G I Bell, C Bogardus, P A Permana

Affiliation

¹ Clinical Diabetes and Nutrition Section, National Institute of Diabetes and Digestive and Kidney Diseases/NIH, 4212 N. 16th Street, Phoenix, AZ 85016, USA.

PMID: 11350192
DOI: 10.1006/mgme.2001.3171

Abstract

The DNA polymorphism SNP-43 in the calpain-10 gene is associated with insulin resistance and reduced skeletal muscle transcript in Pima Indians. Alternative splicing generates transcript isoforms calpain-10a to -10h. We determined the contribution of calpain-10 mRNA isoforms to the decreased total skeletal muscle calpain-10 mRNA levels observed in the G/G homozygotes. The expression levels of the major isoforms, calpain-10a and -10f, were positively correlated with the total calpain-10 mRNA levels, indicating a cumulative effect.

MeSH terms

Calpain / genetics*
Female
Genotype
Humans
Indians, North American / genetics
Isoenzymes / genetics
Male
Muscle, Skeletal / enzymology
Muscle, Skeletal / metabolism*
Polymorphism, Genetic
RNA, Messenger / genetics
RNA, Messenger / metabolism*
Transcription, Genetic

Substances

Isoenzymes
RNA, Messenger
Calpain
calpain 10