Metabolism of the veterinary fluoroquinolone sarafloxacin by the fungus Mucor ramannianus

I A Parshikov; J P Freeman; J O Lay Jr; J D Moody; A J Williams; R D Beger; J B Sutherland

doi:10.1038/sj.jim.7000077

Metabolism of the veterinary fluoroquinolone sarafloxacin by the fungus Mucor ramannianus

J Ind Microbiol Biotechnol. 2001 Mar;26(3):140-4. doi: 10.1038/sj.jim.7000077.

Authors

I A Parshikov¹, J P Freeman, J O Lay Jr, J D Moody, A J Williams, R D Beger, J B Sutherland

Affiliation

¹ National Center for Toxicological Research, Food and Drug Administration, Jefferson, AR 72079, USA.

PMID: 11420653
DOI: 10.1038/sj.jim.7000077

Abstract

To investigate the microbial biotransformation of veterinary fluoroquinolones, Mucor ramannianus was grown in sucrose/peptone broth with sarafloxacin for 18 days. Cultures were extracted with ethyl acetate and extracts were analyzed by liquid chromatography. The two metabolites (26% and 15% of the A280, respectively) were identified by mass and 1H nuclear magnetic resonance spectra as N-acetylsarafloxacin and desethylene-N-acetylsarafloxacin. The biological formation of desethylene-N-acetylsarafloxacin has not been previously observed.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Anti-Infective Agents / metabolism*
Biodegradation, Environmental
Ciprofloxacin / analogs & derivatives*
Ciprofloxacin / metabolism*
Fluoroquinolones*
Mucor / metabolism*
Soil Pollutants / metabolism
Veterinary Medicine / methods

Substances

Anti-Infective Agents
Fluoroquinolones
Soil Pollutants
Ciprofloxacin
sarafloxacin