A very efficient method to cleave Lipid A and saccharide components in bacterial lipopolysaccharides

Carbohydr Res. 2001 Jul 19;333(4):339-42. doi: 10.1016/s0008-6215(01)00157-4.

Abstract

A novel mild procedure for the selective cleavage of ketosidic linkages is developed using ceric ammonium nitrate (CAN) in anhydrous N,N-dimethylformamide. Its application to lipopolysaccharides (LPS) is very significant because in the so far investigated LPS, the connection between the Lipid A region and the oligo(poly)saccharide part is always a keto-sugar. This procedure has been tested on LPS of Escherichia coli which contains Kdo as a linker between Lipid A and OPS and on Acinetobacter haemoliticus which contains D-glycero-D-talo-2-octulopyranosonic acid (Ko) as a linker and it performed efficiently in both cases.

MeSH terms

  • Acinetobacter / chemistry
  • Carbohydrate Sequence
  • Carbohydrates / chemistry*
  • Cerium
  • Dimethylformamide
  • Escherichia coli / chemistry
  • Lipid A / chemistry*
  • Lipopolysaccharides / chemistry*
  • Molecular Sequence Data

Substances

  • Carbohydrates
  • Lipid A
  • Lipopolysaccharides
  • Cerium
  • Dimethylformamide
  • ceric ammonium nitrate