Regions of microsynteny in Magnaporthe grisea and Neurospora crassa

Fungal Genet Biol. 2001 Jul;33(2):137-43. doi: 10.1006/fgbi.2001.1286.

Abstract

A bacterial artificial chromosome (BAC) clone containing 110,467 bp of genomic DNA from Magnaporthe grisea was sequenced, annotated, and compared to the genomes of Neurospora crassa, Candida albicans, and Saccharomyces cerevisiae. Twenty-six open reading frames (ORFs), involved in multiple biochemical pathways, were identified in the BAC sequence. A region of 53 kb, containing 18 of the 26 ORFs, was found to be syntenic to a portion of the N. crassa genome. Subregions of complete colinearity as well as interrupted colinearity were present. No synteny was evident with either C. albicans or S. cerevisiae. The identification of syntenic regions containing highly conserved genes across two genera that have been evolutionarily separated for approximately 200 million years elicits many biological questions as to the function and identity of these genes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Candida albicans / genetics
  • Chromosomes, Artificial, Bacterial
  • Cloning, Molecular
  • DNA Transposable Elements
  • DNA, Fungal
  • Genetic Linkage
  • Genome, Fungal
  • Magnaporthe / genetics*
  • Neurospora crassa / genetics*
  • Open Reading Frames
  • Saccharomyces cerevisiae / genetics
  • Sequence Analysis, DNA
  • Sequence Homology
  • Synteny*

Substances

  • DNA Transposable Elements
  • DNA, Fungal