Abstract
An improved RP-HPLC method was developed for the determination of the configuration and stereochemical purity of cysteine residues in peptides. The method consists of oxidation of cysteine and cystine residues to cysteic acid, followed by hydrolysis and pre-column chiral derivatization with Val-Marfey's reagent.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Chromatography, High Pressure Liquid / methods*
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Cysteic Acid / analysis
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Cysteic Acid / chemistry
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Cysteine / analysis*
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Cysteine / chemistry*
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Fluorescence
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Hydrolysis
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Isomerism
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Molecular Conformation
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Oxidants / metabolism
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Oxytocin / analogs & derivatives
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Oxytocin / chemical synthesis
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Oxytocin / chemistry*
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Peptide Fragments / chemical synthesis
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Peptide Fragments / chemistry*
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o-Phthalaldehyde / chemistry
Substances
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Oxidants
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Peptide Fragments
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Oxytocin
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o-Phthalaldehyde
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Cysteic Acid
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Cysteine