[Amplification, clone and identification of the specific fragments of tumor metastasis-suppressor gene nm23-H1 and nm23-H2]

Hua Xi Kou Qiang Yi Xue Za Zhi. 1997 Nov;15(4):322-4.
[Article in Chinese]

Abstract

A series of DNA primers specific for the specific fragments of nm23-H1 and nm23-H2 were designed and synthesized. The specific fragments of nm23-H1(185 bp) and nm23-H2(145 bp) were amplified from human blood DNA by using polymerase chain reaction (PCR). The recovered PCR products were treated with Klenow fragment, inserted into pGEM-3zf(+) vector with blunt-end ligation, and then transformed into competent cell JM109. The positive colonies were directly identified by colour screening on indicator plates. The recombinant plasmids were digested by Alu I and identified by PCR. The results showed that the authors had obtained the specific fragments of nm23-H1 and nm23-H2 respectively, and these specific fragments could be used for study the expression of nm23-H1 and nm23-H2 seperately.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cloning, Molecular
  • DNA / genetics*
  • Gene Amplification
  • Genes, Tumor Suppressor*
  • Humans
  • Monomeric GTP-Binding Proteins / genetics*
  • NM23 Nucleoside Diphosphate Kinases
  • Nucleoside-Diphosphate Kinase*
  • Polymerase Chain Reaction
  • Transcription Factors / genetics*

Substances

  • NM23 Nucleoside Diphosphate Kinases
  • Transcription Factors
  • DNA
  • NME1 protein, human
  • Nucleoside-Diphosphate Kinase
  • Monomeric GTP-Binding Proteins