Role of endothelin-1 in the active constriction of human atherosclerotic coronary arteries

Circulation. 2001 Sep 4;104(10):1114-8. doi: 10.1161/hc3501.095707.

Abstract

Background: Atherosclerotic coronary arteries are prone to constriction but the underlying causes are incompletely understood. We tested the hypothesis that endothelin-1 (ET-1), a potent vasoconstrictor, contributes to the heightened tone of atherosclerotic human coronary arteries.

Methods and results: In 8 patients with coronary artery disease (CAD) and 8 patients with angiographically smooth coronary arteries (normal), we infused BQ-123, an antagonist of the ET(A) receptor, into a major coronary artery (infused artery) at 40 nmol/min for 60 minutes. The infused artery in the CAD patients contained a >50% stenosis. Using quantitative angiography, we compared the dilation of the infused artery with another, noninfused coronary artery. To estimate the magnitude of the contribution of ET-1 to coronary tone, we compared the dilation to BQ-123 with that elicited by intracoronary nitroglycerin (200 microgram). BQ-123 induced significant dilation in the normal arteries (7.3% at 60 minutes, P<0.001 versus noninfused arteries) and a greater dilation in the CAD arteries (16.3% at 60 minutes, P<0.001 versus infused normal arteries). The dilation at stenoses was particularly pronounced (21.6% at 60 minutes, P<0.001 versus infused CAD arteries). Compared with the dilation from nitroglycerin, ET-1 contributed to 39% of the coronary tone in normal arteries, 74% of tone in CAD arteries, and 106% of tone at stenoses (P<0.01).

Conclusions: ET-1 accounts for nearly all the resting tone in atherosclerotic coronary arteries, especially at stenoses. Inhibitors of ET-1, by relieving constriction, may significantly lessen the hemodynamic significance of coronary stenoses and thereby reduce myocardial ischemia.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Coronary Artery Disease / physiopathology*
  • Coronary Circulation / drug effects
  • Coronary Vessels / drug effects
  • Coronary Vessels / pathology
  • Coronary Vessels / physiopathology*
  • Endothelin Receptor Antagonists
  • Endothelin-1 / physiology*
  • Humans
  • Middle Aged
  • Multivariate Analysis
  • Nitroglycerin / pharmacology
  • Peptides, Cyclic / pharmacology
  • Receptor, Endothelin A
  • Vasoconstriction / drug effects
  • Vasoconstriction / physiology*
  • Vasodilator Agents / pharmacology

Substances

  • Endothelin Receptor Antagonists
  • Endothelin-1
  • Peptides, Cyclic
  • Receptor, Endothelin A
  • Vasodilator Agents
  • Nitroglycerin
  • cyclo(Trp-Asp-Pro-Val-Leu)