Reduced release of DNA from streptococcus pneumoniae after treatment with rifampin in comparison to spontaneous growth and ceftriaxone treatment

Eur J Clin Microbiol Infect Dis. 2001 Jul;20(7):490-3. doi: 10.1007/pl00011291.

Abstract

In order to study the release of DNA from Streptococcus pneumoniae in vitro during spontaneous growth and treatment with ceftriaxone or rifampin, a semiquantitative polymerase chain reaction was used. During spontaneous growth, high concentrations of bacterial DNA were released. Exposure to 10 microg/ml of ceftriaxone decreased the DNA release, in median, by 19 times (P=0.03 vs. spontaneous growth). Treatment with 10 microg/ml of rifampin led to a reduction of DNA release, in median, by a factor of 49 (P=0.03 vs. ceftriaxone; six experiments performed on different days).

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Ceftriaxone / pharmacology*
  • DNA, Bacterial / analysis*
  • DNA, Bacterial / drug effects*
  • Humans
  • Immunoenzyme Techniques
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Probability
  • Reference Values
  • Rifampin / pharmacology*
  • Sensitivity and Specificity
  • Streptococcus pneumoniae / drug effects*
  • Streptococcus pneumoniae / growth & development*

Substances

  • DNA, Bacterial
  • Ceftriaxone
  • Rifampin