2'-modified oligonucleotides from methoxyoxalamido and succinimido precursors: synthesis, properties, and applications

Nucleosides Nucleotides Nucleic Acids. 2001 Apr-Jul;20(4-7):507-14. doi: 10.1081/NCN-100002326.

Abstract

Synthesis of 2'-modified oligonucleotides from 2'-methoxyoxalamido (MOX) and 2'-succinimido (SUC) precursors is described. Their physical and biochemical properties were assessed. Synthesized oligonucleotides were used as primers in advanced DNA sequencing protocols. An example of sequencing directly off genomic DNA template without prior cloning or PCR amplification is presented.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • DNA Primers / chemical synthesis*
  • DNA Primers / chemistry
  • Molecular Sequence Data
  • Oligonucleotides / chemical synthesis*
  • Oligonucleotides / chemistry
  • Oxamic Acid / analogs & derivatives*
  • Oxamic Acid / chemistry
  • Phosphorylation
  • Sequence Analysis, DNA / methods
  • Succinimides / chemistry*

Substances

  • DNA Primers
  • Oligonucleotides
  • Succinimides
  • Oxamic Acid