Suppression subtraction hybridization and northern analysis reveal upregulation of heat shock, trkB, and sodium calcium exchanger genes following global cerebral ischemia in the rat

Brain Res Mol Brain Res. 2001 Sep 30;93(2):173-9. doi: 10.1016/s0169-328x(01)00203-0.

Abstract

Driver (sham-operated) and tester (ischemic) hippocampal cDNAs were subtracted, and the resulting ischemia-induced upregulated gene expression was verified by northern analysis. cDNAs isolated corresponded to (1) genes known to be upregulated following ischemia, (hsc70, hsp90, hsp105 and trkB) and (2) a gene not previously implicated with cerebral ischemia, sodium calcium exchanger (ncx). Furthermore, upregulation of these genes was demonstrated following preconditioning transient global ischemia.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Northern
  • Brain Ischemia / genetics
  • Brain Ischemia / metabolism*
  • DNA, Complementary / genetics
  • Gene Expression Profiling* / methods*
  • Gene Expression Regulation*
  • Heat-Shock Proteins / biosynthesis*
  • Heat-Shock Proteins / genetics
  • Hippocampus / metabolism*
  • Ischemic Attack, Transient / genetics
  • Ischemic Attack, Transient / metabolism
  • Male
  • Nerve Tissue Proteins / biosynthesis*
  • Nerve Tissue Proteins / genetics
  • Rats
  • Rats, Sprague-Dawley
  • Receptor, trkB / biosynthesis*
  • Receptor, trkB / genetics
  • Sodium-Calcium Exchanger / biosynthesis*
  • Sodium-Calcium Exchanger / genetics
  • Subtraction Technique
  • Up-Regulation*

Substances

  • DNA, Complementary
  • Heat-Shock Proteins
  • Nerve Tissue Proteins
  • Sodium-Calcium Exchanger
  • Receptor, trkB