Abstract
Driver (sham-operated) and tester (ischemic) hippocampal cDNAs were subtracted, and the resulting ischemia-induced upregulated gene expression was verified by northern analysis. cDNAs isolated corresponded to (1) genes known to be upregulated following ischemia, (hsc70, hsp90, hsp105 and trkB) and (2) a gene not previously implicated with cerebral ischemia, sodium calcium exchanger (ncx). Furthermore, upregulation of these genes was demonstrated following preconditioning transient global ischemia.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Blotting, Northern
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Brain Ischemia / genetics
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Brain Ischemia / metabolism*
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DNA, Complementary / genetics
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Gene Expression Profiling* / methods*
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Gene Expression Regulation*
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Heat-Shock Proteins / biosynthesis*
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Heat-Shock Proteins / genetics
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Hippocampus / metabolism*
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Ischemic Attack, Transient / genetics
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Ischemic Attack, Transient / metabolism
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Male
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Nerve Tissue Proteins / biosynthesis*
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Nerve Tissue Proteins / genetics
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Rats
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Rats, Sprague-Dawley
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Receptor, trkB / biosynthesis*
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Receptor, trkB / genetics
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Sodium-Calcium Exchanger / biosynthesis*
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Sodium-Calcium Exchanger / genetics
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Subtraction Technique
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Up-Regulation*
Substances
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DNA, Complementary
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Heat-Shock Proteins
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Nerve Tissue Proteins
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Sodium-Calcium Exchanger
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Receptor, trkB